OBJECTIVES To review evidence about the joint relation of exposure to asbestos and smoking on the risk of lung cancer to answer three questions: (1) does asbestos increase risk in non-smokers; (2) are the data consistent with an additive model; and (3) are the data consistent with a multiplicative model?
METHODS Analysis of 23 studies reporting epidemiological evidence on the joint relation. Comparison of risk of lung cancer in subjects unexposed to asbestos or smoking, exposed to asbestos only, to smoking only, or to both. Estimation of the relative risk associated with asbestos exposure in non-smokers and of statistics testing for additivity and multiplicativity of risk.
RESULTS Eight of the 23 studies provided insufficient data on the risk of lung cancer in non-smokers to test for possible effects of asbestos. Asbestos exposure was associated with a significantly (p<0.05) increased risk in non-smokers in six of the remaining studies and with a moderately increased, but not significant, increase in a further six. In two of the three studies that found no increase, asbestos exposure was insufficient to increase risks in smokers. In 30 of 31 data sets analysed, risk in the combined exposure group was greater than predicted by the additive model. There was no overall departure from the multiplicative model, the proportional increase in risk of lung cancer with exposure to asbestos being estimated as 0.90 (95% confidence interval (95% CI) 0.67 to 1.20) times higher in smokers than non-smokers. For two studies significant (p<0.05) departures from a multiplicative relation were found in some, but not all, analyses. Reasons are presented why these may not indicate true model discrepancies.
CONCLUSIONS Asbestos exposure multiplies risk of lung cancer by a similar factor in non-smokers and smokers. The extent to which it multiplies risk varies between studies, no doubt depending on the type of asbestos involved, and the nature, extent, and duration of exposure.
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Lung cancer incidence is clearly increased by both smoking and exposure to asbestos, but the joint relation is not well defined. Suppose that risks are 1 unit for a non-smoker unexposed to asbestos, A units for a non-smoker exposed to asbestos, and S units for an unexposed smoker. Various possible models predict risk in a smokers exposed to asbestos. In the additive model the excess risks add to give a predicted risk of 1+(A-1)+(S-1)=A+S-1 units. In the multiplicative model the two proportional increases multiply to give AS units. In the intermediate model the risk lies between A+S-1 and AS units; in the supermultiplicative model it exceeds AS. Note that to test the models only requires data on the relative, not absolute, risks and also that the models, if correct, should apply equally to risk differences relating to high versus low exposure as to those relating to exposed versus unexposed.
We examine studies investigating the relation of lung cancer to both agents to answer three questions:
(1) Does asbestos increase lung cancer risk in non-smokers?
(2) Do the data fit an additive model, or is the absolute risk increase from asbestos greater for smokers than non-smokers? and
(3) Do the data fit a multiplicative model, or does the relative risk increase from asbestos vary by smoking?
Other published reviews1-7are not comprehensive and do not answer all these questions clearly.
Relevant papers were obtained from in house files, additional papers identified from Medline and Embase searches, and papers cited as references. Studies had to report evidence on the joint relation of smoking and asbestos to lung cancer. One study8 concerning location and histology of lung cancer, but not risk itself, was excluded.
Study details extracted included its location, timing, and design, the number of lung cancers, how they were diagnosed, and how asbestos exposure and smoking were defined. To test the various hypotheses, subjects were categorised into four groups: unexposed to asbestos or smoking (A-S-), or exposed to asbestos only (A+S-), smoking only (A-S+), or both (A+S+). Sometimes A- included low exposure to asbestos and S- included light smoking.
Summary statistics allowing comparison of risk in the four groups were extracted or calculated.9 For case-control studies, these were numbers of cases and controls by group and estimates, derived from odds ratios, of risk relative to A-S- (or to A+S- if no cases in A-S-). For cohort and occupational studies, relative risks were derived from standardised mortality ratios (SMRs), incidence ratios (SIRs), or lung cancer rate estimates. In some cohort and occupational studies (group A) internal data were available for all four groups. In others (group B), where the whole population was considered to be exposed to asbestos (A+) and comparisons were made to an external standard, SMRs/SIRs were only available for A+S- and A+S+. Here relative risks were calculated assuming (from 40 year follow up data for male British doctors10) that the relative risk for smoking in those unexposed to asbestos was 7.13, with the SMR 0.15 for A-S- and 1.07 for A-S+. Sensitivity analyses were also carried out with alternative smoking risk estimates of 3, 5, and 10. Where expected (E) numbers of lung cancers were presented to a common reference, SMRs/SIRs were calculated from the observed (O)/expected (E) ratio. Where expected numbers provided were adjusted for smoking (ES), SMRs were calculated by multiplying O/ES by 0.15 for S- and by 1.07 for S+, again from the data from British doctors.
Where data permitted, study specific estimates were made of the relative risk from asbestos in non-smokers, and of statistics testing for additivity, U, and multiplicativity,V. U=R1-R2-R3+R 4andV=R1R4/R2R 3where R1, R2, R 3 and R 4 are the relative risks in, respectively, groups A-S-, A+S-, A-S+, and A+S+. 95% Confidence intervals (95% CIs) of the asbestos risk in non-smokers and of V were calculated assuming the relative risk is log normally distributed.
Multiplicativity was further tested by fitting logistic or log linear models, and by fixed effects meta-analyses11 ofV. Percentage attributable risks (PARs) were estimated for each study based on the relative risks fitted to the multiplicative model (F1,F2,F3,F 4) and the population distribution of exposure.2 12Thus, deaths were divided as in table 1.
Tables 2-4 summarise characteristics of, respectively, nine case-control studies (including two nested within an occupational study),13-23 seven group A cohort and occupational studies,1 24-29and seven group B cohort and occupational studies.30-36Twelve studies were conducted in Europe, seven in North America, three in Asia, and one in Australia.
Four studies were conducted in miners (and millers), two of chrysotile,15 24 one crocidolite13 and one anthophyllite asbestos.28 Seven were conducted in asbestos products workers, two of chrysotile,25 26 two amosite,27 34 one crocidolite and chrysotile1 and two of unstated asbestos type.30 33 One study was of workers in a nitric acid production plant,29 one of asbestos sprayers and of patients with asbestosis and silicosis,31 and three of insulation workers.32 35 36 The remaining seven studies were case-control, in railway workers,20 industrial areas,16 and shipbuilding areas.14 17-19 21-23
Of the 16 occupational cohort studies, four started follow up in the 1940s, four in the 1950s, five in the 1960s, and three in the 1970s. Follow up ranged from 9–42 years. For many studies with follow up starting early, smoking habits were obtained later, limiting numbers of lung cancers where analysis by smoking was possible. Fourteen occupational cohort studies reported results for lung cancer mortality, based on death certificates only in eight and also based on medical records in six. The other two reported cancer incidence. Of seven (non-nested) case-control studies, two involved hospital patients, three dead cases, and two both. One case-control study used general population controls. The rest used hospital controls for hospital cases and dead controls for dead cases, with varying exclusions used for controls (table2).
Numbers of lung cancers with available data about smoking varied, from at most 50 in eight studies to about 1000 in two. Many larger studies involved few cases with severe asbestos exposure. The studies of chrysotile miners and millers in Quebec24 and of insulation workers in the United States and Canada36involved the most severely exposed cases. Many studies had few cases, so lacking power to detect asbestos risks in non-smokers.
DATA ON ASBESTOS AND SMOKING
Tables 5-7 define exposures and present relevant data. Generally, the studies considered correspond to those described in tables 2-4respectively. However, in four studies1 24 27 28 table6 shows analyses comparing risk of high and low asbestos exposure within the study and table 7 shows analyses of high exposure with an external standard.
Definitions and sources of asbestos exposure varied. In some studies in table 7 36 the risk of the whole population was compared with an external standard, exposure being inferred from the occupation. In most occupational and cohort studies, subjects were categorised into high or low exposure based on work history, sometimes supplemented by dust measurements. For case-control studies evidence of exposure was usually derived from work history obtained from various sources (work records, interview of patient, interview of proxy). In the studies by Blot et al,21-23 data were collected only on shipyard employment.
Data on smoking were obtained by proxy interview in over a third of studies. Although some studies separated results for ever and never smokers, the S- category often included light smokers, ex-smokers, or non-cigarette smokers.
Generally, age was taken into account in design or analysis, but other environmental causes of lung cancer were not (data not shown). However, the data of Pastorino et al 16were subdivided by exposure to polycyclic aromatic hydrocarbons, Garshick et al 20 considered diesel exposure, and Selikoff et al 34 and Hammond et al 36 sought comparability of the study and reference group by calculating expected values for United States white men who were not farmers, had at most high school education, and had been occupationally exposed to dust, fumes, vapours, gases, chemicals, or radiation.
Some risk estimates shown in tables 5-7 are based on few lung cancers. For some studies, not all the required data were estimable.
ANSWERING THE THREE QUESTIONS OF INTEREST
The data generally showed a clearly increased risk of lung cancer with smoking (ignoring asbestos), and with asbestos (ignoring smoking).
Only three studies failed to show a significantly increased risk of lung cancer with asbestos for the whole population. The study of railway workers20 and the study of asbestos cement workers30 showed little or no relation, whereas the study of Italian chrysotile miners and millers15 reported a relative risk of 2.89 (95% confidence interval (95% CI) 0.58 to 14.4) for high to low exposure, based on only 12 lung cancers.
DOES ASBESTOS INCREASE THE RISK OF LUNG CANCER IN NON-SMOKERS?
Rubino et al 15 provided no useful information, as no lung cancers occurred in non-smokers and comparisons were only made within the study. Nor did Minowaet al 18 because of inadequate reporting. The other 21 studies can be considered as four sets. The first showed a significantly increased relative risk (in at least one analysis) of 25.0,34 11.5,18.44,35 5.71,36 4.07,24 and 3.78.26 The second reported at least a moderate increase which was not significant (or significance could not be tested). Relative risks were 5.44,25 2.52,162.41,19 1.90,13 1.83,17 and 1.28, 1.88, and 1.80 (three United States states).21-23The third showed little evidence of an effect of asbestos in non-smokers. Neuberger and Kundi30 and Garshicket al 20 have already been noted to show little evidence of an effect overall, whereas Martischniget al 14 had wide 95% CIs of 0.38 to 3.06. The final set had virtually no power.27-29 31-33
The evidence clearly indicates that, provided exposure is sufficient to increase risk in the overall population, and enough non-smokers are studied, an increased risk of lung cancer in non-smokers after exposure to asbestos can be shown. The magnitude of the increase depends on the extent and nature of exposure.
DO THE DATA FIT AN ADDITIVE MODEL?
For an additive model, the sum of risks for A-S- and A+S+ should equal that for A+S - and A-S+. Tables 8-10 present a statistic (U) testing additivity based on the difference of the sums. Overall, there are 31 estimates ofU, 30 indicating that the response is higher than expected for A+S+ (p<0.001). Some studies show very clear departures from additivity, notably,36where the increased risk from asbestos in smokers is reliably estimated at about 40 times the baseline risk. Had the increase in non-smokers been 40 times the baseline risk, about 60 lung cancer deaths would have been expected in the non-smoking workers, but only eight were found. Other studies show quite clearly non-additive results.19 29 31 34
DO THE DATA FIT A MULTIPLICATIVE MODEL?
For a multiplicative model, the product of risks for A-S- and A+S+ should equal that for A+S- and A-S+. Tables 8-10 present a statistic (V) testing multiplicativity, based on the ratio of the products. V is not obviously consistently greater or less than 1.0. Restricting attention to table 9rather than table 10 estimates ofV,1 24 27 28 and excluding undefined or infinite estimates (all based on few cases), meta-analysis of 16 individual estimates gave an estimate of 0.90 (95% CI 0.67 to 1.20), with no significant heterogeneity between estimates.
Tables 8-10 also include results of formal model fitting, showing estimated risks for asbestos exposure alone (A), smoking alone (S) and their product (AS), as well as the deviance, distributed approximately as χ2 on 1 degree of freedom.
Based on the deviances (or, equivalently, on the 95% CIs of V), only four estimates show any indication of departure from multiplicativity.
Chance also cannot be excluded for the analysis of Berryet al 1 for women for 1971–80 (table 10), where the deviance was 3.51. The analysis compares an observed relative risk of smoking of 2.26 in female with a value of 7.13 for male British doctors. Use of a lower reference eliminates the discrepancy.
For the data of McDonald et al,24 two analyses were conducted. The first (table9) tests if the relative increase in risk for smoking was similar in men with high and low exposure to asbestos. Although less in those with high exposure (2.73) than with low exposure (4.46) the difference was not significant. The second (table 10) tests if the relative increase in risk from smoking in men with high exposure to asbestos was similar to that for British doctors. The difference (2.73) versus (7.13) was significant. The first analysis avoids assuming that smoking habits of British doctors and Quebec miners are similar and the problem that pipe and cigar smoking were only accounted for in the study of doctors. The data from the study by McDonald et al do not clearly show true departure from the multiplicative model.
The largest deviance (15.85, p<0.001) occurred for the study of Selikoff et al (table 10).34Forty five deaths occurred from lung cancer, among ever smokers of cigarettes, compared with 9.6 expected for men with the same smoking history in the American Cancer Society's million people study, and five deaths occurred as against 0.2 expected among men who had never smoked, V being estimated as 0.19 (95% CI 0.07 to 0.61). This analysis was based on death certificate diagnoses to make it comparable with the reference population. However, based on the “best available evidence”, there were 55 deaths from lung cancer among smokers and three among non-smokers.13 34 With these numbers,V becomes a non-significant 0.38 (95% CI 0.12 to 1.91). Although the revised analysis ignores misdiagnosis in the reference population, it casts doubt on whether the data of Selikoff et al truly misfit.34
Overall, the available data fit the multiplicative model well.
Tables 8-10 also include fitted values of the risks for asbestos only, smoking only, and both exposures combined. Those for asbestos only are very variable, generally smaller for case-control studies than others. Virtually all estimates are greater than unity confirming that exposure to asbestos increases risk of lung cancer. The variation reflects differences in extent and type of exposure to asbestos between the populations studied. All estimated risks of smoking are greater than unity. The variation reflects differences in definitions of smoking used in different studies and in smoking history for the different populations. The estimated risks for joint exposure vary, from about four in the studies by McDonald et al 24 and Berry et al(1971-80, men)1 to over 50 in the studies of Hammondet al,36 Selikoffet al,35 and Oksaet al 31 (asbestos sprayers and asbestosis patients).
Conclusions from group B studies were generally independent of the assumed relative risk of smoking of 7.13 for populations unexposed to asbestos. With alternative values of 3, 5, or 10, all those studies in table 10 showing an increased risk associated with exposure to asbestos in non-smokers continued to do so, and all the studies that showed a clearly non-additive pattern of results also continued to do so. The only study where the choice of reference risk affected conclusions about multiplicativity was that of McDonald et al, as already discussed.24
Based on the fitted multiplicative model estimates, PARs for background exposure to asbestos only, smoking only, and their joint effect were calculated, firstly among those exposed to both agents, and secondly among the whole population studied. Table 11 presents means of these estimates separately by study type. The PAR estimates varied considerably between studies (data not shown) due to differences between studies in the extent of exposure to asbestos and in the definition of the smoking categories. For group B cohort—occupational studies where the estimated effect of asbestos was relatively high—the PAR for background was relatively low and that for joint exposure relatively high. The PAR for asbestos only was also similar to that for smoking only, whereas in case-control and group A studies it was substantially lower.
LIMITATIONS OF THE EVIDENCE
Effects of asbestos are difficult to study, some studies representing years of dedicated work. However, various limitations affect many or all of the studies, including: (a) Reliance on death certificate data, known to be inaccurate37 (for studies with an external reference this is inevitable, the reference data being based on death certification); (b) difficulties in assessment of exposure to asbestos, which is often only an educated guess (dust measurements, even where available, are never complete); (c) inaccuracies in smoking history, no studies validating self reported non-smoking by cotinine measurements; (d) inconsistent classification of smoking, with the unexposed group often including light smokers, ex-smokers, or non-cigarette smokers; (e) failure to account for other factors associated with lung cancer; (f) reliance on data obtained from proxy respondents; and (g) small numbers of lung cancers.
Three limitations seem most serious. Firstly, the sparsity of lung cancers in some studies leads to unreliable risk estimates, particularly in non-smokers.
Secondly, failure to account for confounding by other lung carcinogens means increased risks in groups that are exposed to asbestos may not result totally from asbestos. For example, some miners may have high radon exposure, railway workers may have increased exposure to coal dust and diesel, and shipyard work may involve exposures other than asbestos.
Thirdly, misclassification of some current or ex-smokers as non-smokers may affect estimates of the effect of asbestos in non-smokers. However, provided a true multiplicative relation exists, it should still be seen after misclassification of smoking, if the misclassification is independent of exposure to asbestos. (Misclassification of exposure to asbestos, which is independent of smoking habits, will also not upset a multiplicative relation.)
Provided a multiplicative relation exists, it should also still be seen regardless of the smoking definition used in a particular study. However, differences in definition will affect the magnitude of the estimated effect of smoking and the proportion of deaths attributed to smoking and its interaction with asbestos.
A true multiplicative relation may not be observed exactly in practice because average exposure to asbestos may differ between non-smokers and smokers exposed to asbestos (or, conversely, because the average amount smoked may differ between smokers exposed and unexposed to asbestos). The model fitting conducted is based on data subdivided into four groups. In principle, it is better to conduct a regression analysis including terms for extent and duration of smoking and of exposure to asbestos and then see whether additional interaction terms are significant, so implying inadequacy of the multiplicative model. Data from the study by Garshick et al were in fact analysed in this way,20 with no interaction detected.
In 1977, Saracci3 reviewed five studies, and concluded that the multiplicative model was “more plausible” than the additive model, although the data “do not allow a definitive discrimination”. Later, Berry et al suggested that the relative risk of lung cancer from asbestos might be six times higher for non-smokers than smokers, but noted “uncertainty on the accuracy of this figure because of possible biases and sampling variation.”
Steenland and Thun6 in 1986, considered that only four studies1 24 35 36 provided sufficient information to evaluate interaction and that the data were “contradictory”. For the study by Berry et al, their conclusion of departure from multiplicativity and also of no departure from the additivity disagrees both with our analyses and those by the original authors.1
An updated review by Saracci4 in 1987 that considered data from 11 studies classified the interaction on a scoring system ranging from “more than multiplicative”, where the risk for A+S+ was at least 25% more than that predicted by the multiplicative model, to “less than additive”, where it was at most 75% of that predicted by the additive model. They noted that “a somewhat variable pattern of interaction has been observed between asbestos and tobacco smoking”, which may “reflect real differences stemming from the fact that both asbestos and smoking act at different stages of the carcinogenic process.” Similar conclusions were reached later by Saracci and Boffetta5 and by Vainio and Boffetta7 based on 13 studies. None of these papers included results of formal tests that the “variable pattern” of interaction actually was significant.
The same is true for the 1999 review by Erren et al,2 which used data from 10 studies reviewed here to estimate the synergy index, the relative excess risk due to interaction, and the PAR due to interaction. The authors concluded that “one-third of cancer cases among smokers who were exposed to asbestos can be attributed to the synergistic behavior of the two carcinogens.” The authors used the terms synergy and interaction as describing departure from an additive model.
Our review clearly shows that exposure to asbestos increases the risk of lung cancer in non-smokers, and that the joint relation of asbestos and smoking to risk is much better described by a multiplicative than by an additive model. The fit to the multiplicative model is generally good, discrepancies noted for two studies (McDonaldet al 24 and Selikoffet al 34) seem to be more apparent than real.
The increased risk from smoking varies by amount of cigarettes smoked, duration of smoking, inhalation, and product smoked, and the definition of the non-smoking denominator used. The increase for asbestos also depends on many factors, not only extent and duration of exposure, but also type of asbestos and nature of exposure. This largely explains why increases in risk for certain occupational groups are larger than for others, although differences in occupational exposures to other carcinogens might contribute.
Multiplicativity implies that attributable risks for smoking and for asbestos may exceed the total risk. Thus, the data of Hammondet al,36 with risk of lung cancer increased about fivefold for asbestos and 10-fold for smoking, taken at face value and ignoring confounding by other exposures, implies that among the insulation workers who smoked, about 90% of their lung cancers could have been avoided by not smoking, and about 80% could have been avoided by not being insulation workers.
I thank John Fry for helpful comments on this review and for assistance in carrying out the statistical tests described. I also thank Pauline Wassell and Diane Morris for their diligent and accurate word processing. Funding for this work was from Philip Morris Europe. The views expressed are those of the author and not necessarily of any other person or organisation.
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