OBJECTIVES To evaluate the association between silica, silicosis and lung cancer, the mortality of 724 patients with silicosis, first diagnosed by standard chest x ray film between 1964 and 1970, has been analysed by a cohort study extended to 31 December 1997.
METHODS Smoking and detailed occupational histories were available for each member of the cohort as well as the estimated lifetime exposure to respirable silica dust and radon daughters. Two independent readers blindly classified standard radiographs according to the 12 point International Labour Organisation (ILO) scale. Lung function tests meeting the American Thoracic Society's criteria were available for 665 patients. Standardised mortality ratios (SMRs) for selected causes of death were based on the age specific Sardinian regional death rates.
RESULTS The mortality for all causes was significantly higher than expected (SMR 1.35, 95% confidence interval (95% CI) 1.24 to 1.46) mainly due to tuberculosis (SMR 22.0) and to non-malignant chronic respiratory diseases (NMCRD) (SMR 6.03). All cancer deaths were within the expected numbers (SMR 0.93; 95% CI 0.76 to 1.14). The SMR for lung cancer was 1.37 (95% CI 0.98 to 1.91, 34 observed), increasing to 1.65 (95% CI 0.98 to 2.77) allowing for 20 years of latency since the first diagnosis of silicosis. Although mortality from NMCRD was strongly associated to the severity of radiological silicosis and to the extent of the cumulative exposure to silica, SMR for lung cancer was weakly related to the ILO categories and to the cumulative exposure to silica dust only after 20 years of lag interval. A significant excess of deaths from lung cancer (SMR 2.35) was found among silicotic patients previously employed in underground metal mines characterised by a relatively high airborne concentration of radon daughters and among ever smokers who showed an airflow obstruction at the time of the first diagnosis of silicosis (SMR 3.29). Mortality for lung cancer related to exposure was evaluated with both the Cox's proportional hazards modelling within the entire cohort and a nested case-control study (34 cases of lung cancer and 136 matched controls). Both multivariate analyses did not show any significant association with cumulative exposure to silica or severity of silicosis, but confirmed the association between mortality for lung cancer and relatively high exposure to radon, smoking, and airflow obstruction as significant covariates.
CONCLUSIONS The findings indicate that the slightly increased mortality for lung cancer in this cohort of silicotic patients was significantly associated with other risk factors—such as cigarette smoking, airflow obstruction, and estimated exposure to radon daughters in underground mines—rather than to the severity of radiological silicosis or to the cumulative exposure to crystalline silica dust itself.
- crystalline silica
- lung cancer mortality
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The relation between silica, silicosis, and lung cancer has been debated for a long time and, currently, still is debated. In 1987, the International Agency for Research on Cancer (IARC)1 2classified crystalline silica as a probable lung carcinogen in humans.
However, some editorials and reviews suggested that smoking and other occupational exposures (polycyclic aromatic hydrocarbons, radon, asbestos, and arsenic) could be confounding factors and crystalline silica could be only a possible promoter, enhancing the carcinogenic effect of cigarette smoking or of other carcinogens.3-7In 1997, the IARC reviewed the body of epidemiological literature and stated that there was sufficient evidence for carcinogenicity of crystalline silica in humans,8 even if some difficulty in reaching this decision has been reported.9
In a recent review10 some of the main scientific uncertainties in the epidemiological evidence on the carcinogenicity of silica and silicosis were emphasised: a direct effect of silica exposure or secondary effect of silicosis; confounding by other exposures, smoking, socioeconomic class difference, and inappropriate reference population; selection and diagnostic bias among cohorts of registered silicotic patients; evidence for exposure-response lacking; and different health effects between quartz and cristobalite.
More recently an extensive re-examination of the literature on this topic was published by Hessel et al.11 The review focused on methodologically correct studies, including some omitted by IARC, about exposure-response analysis of workers exposed to crystalline silica and studies of workers with silicosis diagnosed at necropsy or radiographically. Contrary to the IARC statement, the authors, who followed standard criteria to assess the evidence of causality,12 concluded that the body of epidemiological literature argues against the carcinogenicity of crystalline silica and that a casual association between severity of silicosis and lung cancer is not world wide. This was supported by studies in which silicotic patients were not identified only from compensation lists.
In 1991, Carta et al 13 assessed the mortality of all Sardinian silicotic patients radiographically diagnosed between 1964 and 1970 followed up to December 1987. Compared with the regional mortalities, the SMR for lung cancer was 1.29 (95% confidence interval (95% CI) 0.85 to 1.96), without significant trend by severity of radiological silicosis. In the nested case-control study (22 cases of lung cancer and 88 controls) analysis by International Labour Organisation (ILO) radiographic category or cumulative exposure to respirable silica (gh/m3) showed no association with lung cancer, the risk estimate for lung cancer being significantly associated with the severity of airflow obstruction, even after adjustment for cigarette smoking.
The present paper extends this previous study with 10 years more of follow up and it has been implemented by an appropriate estimation of exposure of silicotic miners and non-miners to radon daughters.
Subjects and methods
Details on subjects and exposure estimates are reported elsewhere.13 14 From about 2000 workers investigated in our Institute in Sardinia, Italy for awarding compensation, the cohort included 724 silicotic patients who had a radiographically confirmed diagnosis between 1964 and 1970. The original chestx ray films, reviewed by two independent blinded readers, were classified according to the ILO 12 point classification scale of profusion,15 assigning a midpoint category when a disagreement between readers was present. However, the variability between readers never exceeded two to three points of the ILO scale.
Of the 724 radiographs showing a silicotic picture, 154 (21.3%) were classified within the categories 1/0 and 1/2, 205 (28.3%) within the categories 2/1 and 2/3, and 365 (50.4%) as having a nodule profusion 3/2 or higher. This included 64 films that also had some large opacities. Information on smoking habits was available for all subjects from the clinical records: 253 men (35.1%) were never smokers and 467 (64.9%) ever smokers of whom 106 (22.6%) had smoked on average 15 or more cigarettes a day. All original spirometric tracings were revised according to the American Thoracic Society criteria16 and an acceptable test was obtained for 665 patients (91.9% of the whole cohort). An airflow obstruction (measured as percentage of forced expired volume in 1 second divided by vital capacity (FEV1/VC%) lower than the normal limits derived from the reference summary equations of the European Coal and Steel Community)17 was found in 307 subjects (46.2%).
ASSESSMENT OF EXPOSURE TO SILICA DUST AND RADON PROGENY
Full employment details on individual jobs and tasks were available for the whole cohort. All subjects were employed for their lifetime only in Sardinian mines and quarries. Only 23 subjects of the entire cohort were still at work at the beginning of the follow up.
Four hundred and thirty two men (59.9%) had worked in underground lead and zinc mines in which the silica concentration in respirable dust ranged between 2% and 29%. Ninety nine were miners from metal mines of the south west of Sardinia characterised by low airborne silica concentration (median 3%) in respirable dust, the wall rocks being dolomite and limestone with a mainly carbonatic matrix. In the west of Sardinia 333 subjects had worked in mines with high silica content (median 13%) in respirable dust, the wall rocks being metamorphic schists with a quartz and syderite matrix. In the Sardinian brown coal (lignite) mines, 116 patients (16.0%) had worked where the quartz content in respirable dust ranged between 2% and 10%, and 176 patients (24.3%) were granite quarrymen (range of silica concentration in respirable dust 5%–25%).
Previous studies18-20 and several environmental dust measurements, available since 1945 from the mining companies, provided findings for the industrial hygienists to retrospectively estimate the median exposure to respirable dust (mg/m3) for each job and task underground and at the surface, stratified by 5 year periods since the 1920s. Estimates were mainly based on available environmental measurements, production/year, mining technologies, and changes in ventilation and in drilling devices. An individual cumulative index of exposure to respirable dust was then calculated by summing the arithmetical products between each median dust concentration assigned to each job and task for each 5 year period and the estimated hours of exposure/year of work experienced by each subject in each workplace. The index has been reported as gh/m3 13 20 21 and categorised by tertiles: ⩽150, 151–250, and >250 gh/m3, the tertiles comprising 316 (43.6%), 270 (37.3%), and 138 (19.1%) subjects, respectively. At the same time, based on the median quartz weight concentration in respirable dust, a cumulative index of exposure to respirable crystalline silica was also calculated in gh/m3. Three categories of exposure were identified: low (⩽5.0 gh/m3), intermediate (5.1–10.0 gh/m3), and high (>10.0 gh/m3), comprising 237 (32.7%), 294 (40.6%), and 193 subjects (26.7%), respectively.
Measurements of underground α radiation produced by radon daughters in the main metal and coal mines in Sardinia were made available only since 1972 by the National Agency for Nuclear and Alternative Energy and by the Institute of Radiological Protection of the National Hydrocarbons Corporation who provided confidential reports to the mining companies.14 In metal mines in the south west of the island the average annual exposure (working time 170 hours/month and 11 months/year) during 1972–88 was 1.4 working level months (WLM)/year (maximum 3.5 WLM/year), with several estimates exceeding the recommended limit of 1.0 WLM/year suggested by NIOSH,22the early exposures to the radon progeny being higher. Cumulative exposures up to 250 WLM were estimated for underground workers employed for a long time in these mines before 1940. In the other lead and zinc mines on the west coast, the exposure to the short lived radon decay products was much lower averaging underground about 0.12 WLM/year, with the exposure concentrations in coal mines being intermediate (median 0.6 WLM/year). Because of the short extent of environmental data we were not able to create careful individual estimates for the radon daughters exposure. However, we have identified 245 subjects of the present cohort (33.8%), firstly engaged in mines before 1940, whose lifetime cumulative exposure to α radiation was estimated as higher than 120 WLM.
Vital status on 31 December 1997 was decided through the roster of compensated silicotic patients of the Insurance Institute and the town residence registers. Twelve men (1.7%), known to be alive on 31 December 1987, were subsequently not traced. They were assumed to be alive at the end of follow up and contributed to person-years at risk up to the end of December 1987. Copies of death certificates were available for all the 579 dead subjects. Causes of death were revised and coded by a trained nosologist according to the ninth revision of the International Classification of Diseases (ICD-9). The standardised mortality ratios (SMRs) and their approximate 95% confidence intervals (95% CIs)23 were calculated for specific causes of death comparing the observed mortality with the expected numbers based on regional rates, available for each 5 year age class and calendar year during the follow up. Trends of SMRs for lung cancer and non-malignant chronic respiratory diseases (NMCRD) by ILO radiological categories of silicosis and by cumulative exposure to respirable dust were tested for significance24 in the whole cohort by different latency periods (10 and 20 years) since the diagnosis of silicosis.
The relation between deaths from lung cancer and potential risk factors for cancer was further examined with Cox's multivariate proportional hazards modelling applied to all members of the cohort with available spirometry (n=665). The Cox's regression program from SPSS for Windows25 was used. The basic time dimension was the individual period of follow up, until death or the end of the time on study. All models were stratified on age at entry (5 year strata). All explanatory variables, except ILO categories, were included as continuous variates (smoking in cigarettes a day, airflow obstruction in the percentage difference between the observed FEV1/VC and the mean predicted ratio, exposure to radon daughters in WLM, and cumulative exposure to respirable silica in gh/m3). The regression coefficients were estimated by the maximum likelihood method and the statistical significance of the influence of each variable was assessed by the likelihood ratio test. The relative risks (RRs) of dying from lung cancer have been calculated for definite values of each variable. To simultaneously control for several factors, stepwise regression analyses (for entry p<0.05) were also performed.
NESTED CASE-CONTROL STUDY
For each subject who died from lung cancer (n=34) a set of four controls was randomly selected from the whole cohort, matched on the year of birth (2 years) and survival of the case.
A conditional stepwise logistic regression program25 was used to study the association between lung cancer and several covariates (ILO categories, smoking, airflow obstruction, and exposure indices) analysed as binary or continuous variables. Calculations were performed on two sets of control patients, those who had or had not died from NMCRD.
Table 1 shows the main characteristics of the whole cohort (724 silicotic patients, 13202 person-years of observation) stratified by ILO categories of radiological silicosis. The mean calendar-year of the first hire and the mean duration of employment in dusty jobs, as well as the mean cumulative exposure to respirable dust were not significantly different between groups with different nodule profusion. On the contrary, an upward trend in severity of radiological silicosis was found for the mean cumulative exposure to respirable silica dust and for the prevalence of an obstructive impairment of lung function. However the frequency of ever smokers was least (60.3%) among patients with nodule profusion 3/2 or greater. Moreover, independent from smoking, airflow obstruction was significantly correlated with the extent of the cumulative exposure to respirable dust.
The overall mortality (table 2) was significantly higher than expected (SMR 1.35, 95% CI 1.24 to 1.46), although all malignant neoplasms were within the expected numbers (SMR 0.93 95% CI 0.76 to 1.14). The mortality risk for malignant neoplasms of the lung (ICD IX 162) was higher than expected (34 observed and 24.9 expected) producing a non-significant SMR of 1.37 (95% CI 0.98 to 1.91) that increased to 1.48 (95% CI 1.02 to 2.16) and 1.65 (95% CI 0.98 to 2.77) after lag intervals of 10 and 20 years since the diagnosis of silicosis. Mortality from tuberculosis was very high (SMR 22.0, 95% CI 17.4 to 27.8) as was mortality from NMCRD, which was six times higher than expected (SMR 6.03, 95% CI 5.44 to 6.69), confirming the well known association between silicosis and these diseases. For 12 silicotic patients the main cause of death was a non-malignant disease of the urinary system (ICD IX 580-599.9), producing an SMR of 1.97 (95% CI 1.13 to 3.43).
Mortality for NMCRD showed significant upward trends in SMRs relative to the severity of radiological silicosis and the cumulative exposure to silica (table 3).
Non-significant trends for mortality from lung cancer relative to the radiological ILO categories and to the cumulative exposure to respirable silica were found in the whole cohort, but were still non-significant even with a latency of 20 years from the diagnosis (table 4).
A significant excess of deaths from lung cancer based on eight observed cancers and 3.4 expected (SMR 2.35, 95% CI 1.20 to 4.61) was found among 99 silicotic patients (person-years 1572.0) previously employed in underground metal mines which had relatively high airborne concentrations of radon daughters (table 5). Furthermore, a significant upward trend in mortality from lung cancer was found by grouping the industries in increasing rank of exposure to radon daughters, independently from silica content in the respirable dusts.
Cigarette smoking and airflow obstruction were independently strongly related to mortality from lung cancer with significant gradients combining the two risk factors. The highest SMR (3.29, 95% CI 2.23 to 4.83) was found among ever smokers who showed a spirometric obstructive impairment at the time of the first diagnosis of silicosis (data not shown in a table).
Regression coefficients and estimated relative risks of dying from lung cancer derived from the Cox's proportional hazard model are reported in table 6. The number of cigarettes smoked a day, the severity of airflow obstruction, and the cumulative exposure to radon daughters were the only significant explanatory variables for deaths from lung cancer in this study. Relative risks associated with exposure to silica dust and to the severity of radiological silicosis were not significantly different from unity. The coefficient of exposure to radon daughters remained significant at the 0.05 level even when smoking and airflow obstruction were included as covariates in the stepwise model.
The nested case-control study confirmed the significant association between mortality from lung cancer and the estimated cumulative exposure to radon daughters (OR 2.21, 95% CI 1.01 to 4.85 for exposures higher than 120 WLM) also after controlling for cigarette smoking (OR 2.86, 95% CI 1.24 to 6.75, comparing ever smokers with non-smokers) and airflow obstruction (OR 3.86, 95% CI 1.72 to 8.70 for patients with FEV1/VC ratio <85% of mean predicted).
No more improvement in significance of the stepwise model was found including the severity of radiological silicosis (OR 1.06, 95% CI 0.80 to 4.24 for categories 3/2+) or the cumulative exposure to respirable silica dust (OR 1.30, 95% CI 0.71 to 2.68 for exposures higher than 10 gh/m3). Results did not change when smoking, airflow obstruction, and exposure to silica and radon were analysed as continuous variables.
Using controls who died from NMCRD did not substantially change the results of the nested case-control study.
As in our previous follow up study,13 the findings of this updated study mostly do not show a significantly consistent association between severity of radiological silicosis or cumulative exposure to crystalline silica and lung cancer mortality, after controlling for smoking and other covariates. Tobacco smoking, airflow obstruction, and a relatively high exposure to radon daughters experienced in some underground mines were the most significant predictors of lung cancer in this study.
However, the roughly doubling of risk of lung cancer found for the highest ILO category (SMR 1.91; 95% CI 0.92 to 3.95) and for the highest stratum of cumulative exposure to silica (SMR 2.08 95% CI 0.88 to 4.91) for 20 years of latency (table 4), although not significant, suggest that the role of these variables should not be completely dismissed.
The significant trend found for mortality from NMCRD, mainly silicosis and chronic obstructive lung diseases, by cumulative exposure to respirable silica proves the consistency of the environmental dust measurements and estimates and the validity of the exposure index. Furthermore, a relation between increasing cumulative exposure to respirable silica and increasing severity of radiological silicosis has been found in our study, suggesting that silicosis as a reliable marker of high exposure to crystalline silica.
Human exposure-response studies show that there is a strong relation between cumulative exposure to silica and the risk of silicosis of severity 1/1 or greater26-30 as well as between increasing burden of crystalline silica retained in the lung and increasing pathological grade of silicosis.31 32
Because until 1970 our institute was the only regional referral centre of the National Insurance Institute for Occupational Diseases for a standardised diagnosis of pneumoconiosis among claimants who were workers exposed to dust, the studied cohort includes almost all incident cases of radiologically confirmed silicosis in Sardinia during 1964–70. Moreover, the diagnosis was only based on blinded reanalysis of original radiographs that were compared with the ILO reference chest films and classified according to the ILO categories12unaware of lung function, smoking, and dust exposure. Thus, our cohort seems relatively free from relevant selection or diagnostic bias and represents a well defined group of silicotic patients who had previously experienced very large exposures to silica dust. Full individual work and smoking history was available in details for all cohort members at entry to the follow up so that it was possible to estimate an individual cumulative exposure to dust and radon and to control for confounding by smoking.
Unfortunately we were unable to simultaneously follow up the internal reference group of non-silicotic cases with the same detailed information on smoking and occupational exposure to compare the relative risk of lung cancer found among the two groups.
Coal miners included in this cohort had worked only in a Sardinian mine of lignite,33 a low rank type of coal, with a 2%–10% quartz in the respirable dusts, which yielded a pneumoconiotic radiological picture mainly characterised by rounded opacities, such as silicosis or anthracosilicosis,18 19 completely different from the typical simple coal worker's pneumoconiosis of miners employed in high rank coal mines.
In studies of silicotic patients a roughly doubling of risk of lung cancer has been collectively described.34. However, most of these studies are based only on compensation lists that make interpretation of results difficult because of confounding by smoking, other exposure, respiratory impairment, and uncertainty of diagnosis. Furthermore, in several studies a stronger relative risk of lung cancer and a stronger relation between exposure to silica and lung cancer has been reported among silicotic patients compared with non-silicotic patients.35
Despite some problems with the application of chest radiographs in epidemiological studies (low sensitivity compared with silicosis confirmed at necropsy, variability between readers, disease misclassification), the ILO standardised radiological method, and its classification as nodule profusion, is the only well consolidated method for detecting and categorising silicosis in exposed workers. Results are inconsistent among studies in which silicosis was assessed radiographically, either by only one chest radiograph at the beginning of follow up or by serial radiographs. Some found a significantly higher relative risk of lung cancer in silicotic compared with non-silicotic patients,38-44 whereas others studies13 45-49 found only weak differences in mortality from lung cancer between silicotic and non-silicotic patients,48 contradictory results between different working trades,45 or no association13 46 47 49 between silicosis and lung cancer after controlling for smoking and exposure to silica dust.
Only a few studies have analysed the risk of lung cancer by increasing category of radiological silicosis13 39 49 50 with contradictory results: two of them39 50 found clear gradients for lung cancer, whereas in the others no consistent relation was found between risk of dying from lung cancer and either severity of radiological silicosis or exposure to silica.13 49
The assessment of a dose-response relation, independently of the severity of silicosis, is of critical importance for an association between lung cancer and cumulative exposure to silica. In workers in the diatomaceous earth industry51 exposed to respirable dust with about 10%–25% of cristobalite, an increasing risk for NMRD and for lung cancer was described with an index for increasing cumulative exposure to crystalline silica. Significant gradients were found for NMRD whereas less convincing results were found for lung cancer in a study52 that updated the same cohort. A positive trend was also reported among South African gold miners exposed to quartz,36 but the possibility of a confounding effect from radon daughters was suggested, as the increased intensity of exposure to dust was correlated with a concurrent potentially increased exposure to α radiations underground. However, no consistent association between exposure to respirable dust and lung cancer was found in the absence of silicosis in other recent studies.42 43 45 47 53-55
In the present study, results from both the Cox's proportional hazard modelling and the nested case-control study were comparable. Cigarette consumption was the most significant explanatory variable of lung cancer mortality which had increased. Apart from smoking habits the severity of obstructive impairment as detected at entry had also increased.
After allowing for these variables, a residual significant effect of relatively high cumulative exposure to radon daughters experienced in underground mines still existed.
On the contrary, neither the cumulative exposure to silica dust nor the severity of radiological silicosis entered the stepwise regressions (p>0.05).
The exclusion of controls who died from NMCRD in the nested case-control study would have had the effect of biasing the odds ratios upward for silica and for silicosis relative to lung cancer. The negative results found for both silica and silicosis despite this potential bias, further support our conclusions.
Thus, short lived radon decay products together with smoking could share the role of initiating the carcinogenic process; the cumulative exposure to crystalline silica and the severity of silicosis, by itself, do not seem to be independent risk factors for lung cancer in the present study. These results are similar to those we found in a previous mortality study14 of two cohorts of active metal miners, still at work in 1973 and followed up to the end of December 1988, who experienced different exposure to crystalline silica and radon daughters in two different Sardinian lead and zinc mines.
The exposure to short lived radon decay products, even at relatively low levels, has been suggested as one of the main likely causes of the excess of lung cancer found in several studies of underground miners14 37 42 56 57 or of registered silicotic patients.49
The association between airflow obstruction and increased risk of lung cancer has been supported by several population based studies58-63 and mortality studies of silicotic patients and metal miners.13 14 All these studies showed increased risk of lung cancer, independently of age and smoking, among subjects whose baseline spirometry at entry in the follow up indicated airflow limitation. The exact mechanism underlying this association is still not known, although it has been suggested that lung tissue damage leading to airway obstruction might enhance the effect of carcinogens or might be the expression of the inability to handle an oxidative insult.61 Smoking and chronic exposure to dust and gases cause inflammatory injury to airways with consequent imbalance of inflammatory mediators and humoral protection (oxidant/antioxidant) contemporaneously leading to progressive airway obstruction and clearing impairment of inhaled particles, increasing the residence time of active carcinogens in the lung. Fibrogenic dusts—such as silica—might cooperate by also impairing the lymphatic clearance of particles because of the lymphatic obstruction by macrophages containing silica or silica alone.64 Silicosis, particularly if associated with chronic airways obstruction, is likely to be the worst condition in this context.13
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It has come to the attention of the editors ofOccupational and Environmental Medicine andOccupational Medicine that there has been inadvertent duplicate publication in our two journals of two papers based on the same subject group and which have significant overlap. The references for these two papers are:
Exposure to benzene in urban workers: environmental and biological monitoring of traffic police in Rome. Crebelli R, Tomei F, Zijno A, et al. Occup Environ Med2001;58:165–71. Environmental and biological monitoring of traffic wardens from the City of Rome. Tomei F, Ghittori S, Imbriani M, et al. Occup Med 2001;51:198–203.
We have contacted the Committee on Publication Ethics who suggested the publication of this notice in our journals.
Anne Cockcroft, Editor, Occupational and Environmental Medicine
Jerry Beach, Editor, Occupational Medicine
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