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Determination of Lead in Blood by Atomic Absorption Spectrophotometry
  1. Stig Selander,
  2. Kim Cramér
  1. aMedical Service I and the Department of Hygiene, University of Göteborg, Göteborg, Sweden

    Abstract

    Lead in blood was determined by atomic absorption spectrophotometry, using a wet ashing procedure and a procedure in which the proteins were precipitated with trichloroacetic acid. In both methods the lead was extracted into isobutylmethylketone before measurement, using ammonium pyrrolidine dithiocarbamate as chelator. The simpler precipitation procedure was shown to give results identical with those obtained with the ashing technique. In addition, blood specimens were examined by the precipitation method and by spectral analysis, which method includes wet ashing of the samples, with good agreement. All analyses were done on blood samples from `normal' persons or from lead-exposed workers, and no additions of inorganic lead were made. The relatively simple protein precipitation technique gave accurate results and is suitable for the large-scale control of lead-exposed workers.

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    Footnotes

    • 1 Aided by grants No. B-66-374 and No. B-67-61P-2094-01 from the Swedish Medical Research Council and by a grant from Försäkringsföretaget Folksam, Stockholm, Sweden.

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